Fig. 4: RNF128 in myeloid cells prevents DSS-induced colitis. | Cell Death & Disease

Fig. 4: RNF128 in myeloid cells prevents DSS-induced colitis.

From: RNF128 deficiency in macrophages promotes colonic inflammation by suppressing the autophagic degradation of S100A8

Fig. 4

A Schematic diagram of the bone marrow transplantation experiments. B Rnf128+/+ → Rnf128+/+ (n = 5) and Rnf128−/− → Rnf128+/+ mice (n = 5) were exposed to 2.5% DSS for 7 days. The body weight of mice was measured daily. Statistical data are presented as mean ± SD. *P < 0.05, ***P < 0.001. C Representative images of colons from the mice in (B). Scale bars, 1 cm. D The colon length in (B) was measured. Statistical data are presented as mean ± SD. **P < 0.01. E Representative H&E-staining images of colon sections from mice in (B). Scale bar, 500 μm. F Histopathology score in (E) were measured. Statistical data are presented as mean ± SD. ***P < 0.001. G Representative Ki67 staining images of distal colon tissue sections from the mice in (B). Scale bars, 500 μm. H The Ki67 positive areas in (G) were analyzed. Statistical data are presented as mean ± SD. *P < 0.05. I Representative F4/80 staining images of distal colon tissue sections from the mice in (B). Scale bar = 500 µm. J The F4/80 positive areas in (I) were measured. Statistical data are presented as mean ± SD. ***P < 0.001. K ELISA analysis of IL-1β and TNF-α levels in serum from the mice in (B). Statistical data are presented as mean ± SD. *P < 0.05, **P < 0.01.

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