Fig. 2: The stability of CARM1 is regulated by PSMD14. | Cell Death & Disease

Fig. 2: The stability of CARM1 is regulated by PSMD14.

From: PSMD14-mediated deubiquitination of CARM1 facilitates the proliferation and metastasis of hepatocellular carcinoma by inducing the transcriptional activation of FERMT1

Fig. 2

A Representative confocal microscopy image of IF colocalization analysis of CARM1 and PSMD14 in HCC cell lines. Scale bar, 20 μm. B Endogenous PSMD14 proteins were immunoprecipitated with anti-PSMD14 antibodies and then analyzed by immunoblotting (left panel). Endogenous CARM1 proteins were immunoprecipitated with anti-CARM1 antibodies and then analyzed by immunoblotting (right panel). The IgG antibody was used as the control. C Western blotting assays showed the expression of CARM1 in control and PSMD14-knockdown HCC cells. D CARM1 mRNA expression levels in control and PSMD14-knockdown HCC cells were determined by qRT-PCR. E The protein expression levels of CARM1 were assessed by Western blotting analysis in HCC cells with either empty vector or PSMD14 overexpression. F The mRNA expression levels of CARM1 were assessed by qRT-PCR in HCC cells with either control or PSMD14 overexpression. G Control and PSMD14-knockdown HCC cells were treated with CHX (20 µM) for the indicated times, and endogenous CARM1 protein expression was detected by Western blotting analysis (left). The expression levels of CARM1 were determined by densitometry. The level of CARM1 protein in CHX-untreated cells (0 h) was set to 100% (n = 3; *p < 0.05 and **p < 0.01).

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