Fig. 8: RAD18 facilitates the recruitment of PolĪ· to the DNA damage sites within the nucleus. | Cell Death & Disease

Fig. 8: RAD18 facilitates the recruitment of PolĪ· to the DNA damage sites within the nucleus.

From: MicroRNA-379-5p attenuates cancer stem cells and reduces cisplatin resistance in ovarian cancer by regulating RAD18/PolĪ· axis

Fig. 8

A The ribbon diagram of RAD18, PolĪ·, and ub-PCNA reproduced from PDB. B Protein-protein docking for RAD18-PolĪ·, RAD18- ub-PCNA, and PolĪ·-ub-PCNA was performed using HDOCK. C A positive correlation between RAD18 and PolĪ· expression in female gynecological malignancies was obtained from the CorrelationAnalyzeR tool. D Co-immunoprecipitation of RAD18, ub-PCNA, and PolĪ· was performed using PolĪ· antibody. A representative western blot image validates the interaction between RAD18, ub-PCNA, and PolĪ·. E Immunofluorescence staining of OVCAR3 adherent cells with RAD18 and PolĪ· showed that PolĪ· had reduced nuclear localization in the absence of RAD18 following cisplatin treatment.

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