Fig. 3: Upregulation of TEC-PD-L1 suppressed immune function and proliferation of T cells. | Cell Death & Disease

Fig. 3: Upregulation of TEC-PD-L1 suppressed immune function and proliferation of T cells.

From: Tumor vascular endothelial cells promote immune escape by upregulating PD-L1 expression via crosstalk between NF-κB and STAT3 signaling pathways in nasopharyngeal carcinoma

Fig. 3

A Expression of PD-1 on peripheral blood T cells from healthy individuals and patients with NPC. B Working model illustrating the co-culture system. C Expression of PD-1 on control TCR-T and co-culture TCR-T. D, E, F Statistical plots of the secretion levels of IFN-γ, TNF-α, and Granzyme B in TCR-T cells detected by flow cytometry after different treatments. Control indicates TCR-T co-incubated with control HUVECs, H-E indicates TCR-T co-incubated with HK1-EBV CM-treated HUVECs, C666-1 indicates TCR-T co-incubated with C666-1 CM-treated HUVECs, and PD-L1i indicates incorporation of PD-L1 inhibitor. G, H At killing efficacy target ratios (E:T) of 5:1 (G) and 1:1 (H), CM-treated HUVECs resulted in a decrease in the antitumor capacity of TCR-T. I, J, K, L Secretion of IFN-γ, TNF-α, Granzyme B and Perforin in the supernatants of different co-culture systems. Control indicates supernatants from the co-culture system of control HUVECs with TCR-T, H-E CM indicates supernatants from the co-culture system of HUVECs treated with HK1-EBV supernatant with TCR-T, and C666-1 CM indicates supernatants from the co-culture system of HUVECs treated with C666-1 supernatant with TCR-T. Data are mean ± SD, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, ns, no significance, two-tailed t-test.

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