Fig. 7: PKD1 protects from polyglutamine-induced apoptosis in a cell model of HD.
From: Down-regulation of neuroprotective protein kinase D in Huntington´s disease
A Representative immunoblot of PKDT and p-PKD (S916) in homogenates from neuroblastoma N2a cell line transfected with PolyQ16 or PolyQ94 in combination with p-EF-BOS-GFP (Ctrl) or p-EF-BOS-PKD1-Ca (PKD1-Ca). Levels of β-actin were used as loading control. B Representative confocal microscopy images of GFP, CFP, and cleaved caspase-3 in the neuroblastoma N2a cell line transfected as described in (A). Nuclei were stained with To-Pro. Scale bar: 10 µm. Graph represents the quantification of cleaved caspase-3+ cells relative to GFP/CFP+ cells 24 h post-transfection (n = 25 cells per condition; n = 3 per group). All data are represented as mean ± SEM. **P < 0.01, using one-way ANOVA, followed by Bonferroni’s post-hoc test.
