Fig. 6: TSPYL5 enhances the nuclear membrane aggregation of G3BP1 to facilitate the formation of RanBP2-G3BP1-p53 complex. | Cell Death & Disease

Fig. 6: TSPYL5 enhances the nuclear membrane aggregation of G3BP1 to facilitate the formation of RanBP2-G3BP1-p53 complex.

From: TSPYL5-driven G3BP1 nuclear membrane translocation facilitates p53 cytoplasm sequestration via accelerating RanBP2-mediated p53 sumoylation and nuclear export in neuroblastoma

Fig. 6

A Immunofluorescence analysis showed the increase of G3BP1 on the nuclear membrane in SK-N-SH with TSPYL5 overexpression (blue arrows pointed). Scale bar = 20 μm. B TSPYL5 overexpression increased the binding of G3BP1 to RanBP2. Nuclear proteins are isolated for Co-IP. Data are presented as the mean ± SD from three experiments, ***p < 0.001. C TSPYL5 overexpression increased the binding of RanBP2 to p53. Data are presented as the mean ± SD from three experiments, ***p < 0.001. D Co-IP assays showed the absence of TSPYL5-RanBP2 or TSPYL5-p53 complexes in SK-N-SH cells. Nuclear proteins are isolated for Co-IP. E Co-IP assays with nucleoproteins showed the increase of the binding of RanBP2 and G3BP1 or p53 in SK-N-SH with TSPYL5 overexpression. F Immunohistochemistry assays showed nuclear membrane aggregation of G3BP1 and cytoplasmic p53 sequestration in two cases with abundant TSPYL5 expression (case 1 and case 2), and such a phenotype of G3BP1 and p53 was absent in other two cases showing few TSPYL5 expression (case 3 and case 4). White arrows indicated the nuclear membrane ___location of G3BP1. Scale bar = 10 μm.

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