Fig. 7: The effect of knockdown of KLF2 on proliferation of the self-renewing macrophages.
From: Establishment of bone marrow-derived M-CSF receptor-dependent self-renewing macrophages
a The self-renewing macrophages were transfected with siRNAs indicated, cultured for 2 days, and analyzed for the expression of KLF2 by qRT-PCR. The level of KLF2 is shown, by setting the value of the control (transfection with Cr pool-A siRNA) as 100%. b The self-renewing macrophages were transfected with siRNAs indicated, cultured for 2 days, and subjected to the MTT assay. The number of cells is shown, by setting the value of the control (transfection with Cr pool-A siRNA) as 100% (mean ± SD, n = 3), *p < 0.05. c, d The self-renewing macrophages were transfected with siRNAs indicated, cultured for 2 days, and subjected to the cell cycle analysis. In d, the percentage of cells in the S phase is summarized (mean ± SD, n = 3), *p < 0.05. e, f The self-renewing macrophages were transfected with siRNAs indicated, cultured for 2 days, and subjected to the apoptotic cell analysis. In f, the percentage of cells positive for both Annexin V and 7-AAD is summarized (mean ± SD, n = 3), *p < 0.05.
