Fig. 4: Protein expression analyses. | Cell Death Discovery

Fig. 4: Protein expression analyses.

From: Identification of CD24 as a potential diagnostic and therapeutic target for malignant pleural mesothelioma

Fig. 4

a Western blot analysis showing expression of the indicated proteins in parent cells, NF2-KO cell clones, exogenous NF2 expression/NF2-KO cell clones, p16-KO cell clones, exogenous p16 expression/p16-KO cell clones, DKO cell clones, and exogenous NF2 and p16 expression/DKO cell clones. Cell lysates obtained were used for western blotting analysis to detect protein levels using the specific antibodies listed in Supplementary Table S1. The cell lysates obtained were used for western blotting analysis. GAPDH was used as an internal control. b Quantitation of p-AKT, p70S6K, and c-Jun in MeT-5A and HOMC-B1 cells. p-AKT, p70S6K, and c-Jun expression levels were quantified by measuring the ratio between phosphorylated and total levels. Data are mean ± SEM (n = 3). *P < 0.05, statistically significant difference.

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