Fig. 1: Identification, validation, and characterization of LYPLAL1-AS1 during the adipogenic differentiation of hAMSCs. | Cell Death Discovery

Fig. 1: Identification, validation, and characterization of LYPLAL1-AS1 during the adipogenic differentiation of hAMSCs.

From: Long noncoding RNA LYPLAL1-AS1 regulates adipogenic differentiation of human mesenchymal stem cells by targeting desmoplakin and inhibiting the Wnt/β-catenin pathway

Fig. 1

a Hierarchical clustering of significantly differentially expressed lncRNAs on day 3 after adipogenic differentiation as compared with matched undifferentiated hAMSCs from three donors. The red arrow indicates LYPLAL1-AS1. b Location of lncRNA LYPLAL1-AS1 on chromosome 1. c Rapid amplification of cDNA ends (RACE) for the 5′ end (left) and 3′ end (right) was performed to determine the full length of LYPLAL1-AS1. d qRT-PCR analysis verified the LYPLAL1-AS1 expression profile at the indicated time points after hAMSC adipogenic differentiation. e Fractionation of LYPLAL1-AS1 in hAMSCs followed by qRT-PCR. GAPDH mRNA served as a cytoplasmic control. U1 mRNA served as a nuclear control. f RNA fluorescence in situ hybridization (FISH) assay for LYPLAL1-AS1 in hAMSCs. 18S was the cytoplasmic mRNA control. U6 was the nuclear RNA control. The quantitative data were normalized to GAPDH, n = 3; data are shown as the mean ± SD; scale bars: 50 µm.

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