Fig. 4: TFAM acts as a mediator of ZZW-115-induced ferroptosis.
From: NUPR1 inhibitor ZZW-115 induces ferroptosis in a mitochondria-dependent manner
A Western blot analysis of TFAM expression in MiaPaCa-2 cells with 1.4 µM ZZW-115 in the presence or absence of 1 μM Fer-1 or NAC for 72 h. B Cell viability in TFAM- or control-transfected MiaPaCa-2 cells following treatment with ZZW-115 for 72 h. AUC was calculated by integration. C ATP content in TFAM- or control-transfected MiaPaCa-2 cells following treatment with ZZW-115 for 72 h. D OCR was determined in TFAM- or control-transfected MiaPaCa-2 cells upon 1.4 µM ZZW-115 treatment for 72 h. TMRM fluorescence (E), Mito-ROS level (F), and cellular ROS level (G) were measured in Indicated MiaPaCa-2 cells following treatment with indicated concentration of ZZW-115 for 72 h. H The mitochondrial network in indicated MiaPaCa-2 cells with 1.4 µM ZZW-115 treatment for 72 h. I Western blot analysis of TFAM expression in siNUPR1-RNA-transfected cells. ATP content (J) TMRM fluorescence (K), Mito-ROS level (L), and cellular ROS level (M) were measured in siNUPR1-transfected cells. For each treatment, statistical significance is *P < 0.05, **P < 0.01, ***P < 0.001, ****P ≤ 0.0001 (two-way ANOVA with Sidak correction or Student’s two-tailed unpaired t-test). Data represent mean ± SEM, n = 3 (with technical triplicates).
