Fig. 2: RNA-seq data revealed the genes and pathways associated with CHES1 in TNBC. | Cell Death Discovery

Fig. 2: RNA-seq data revealed the genes and pathways associated with CHES1 in TNBC.

From: Acetylation of Checkpoint suppressor 1 enhances its stability and promotes the progression of triple-negative breast cancer

Fig. 2

A Western blot showed the knockdown efficiency of shCHES1 in BT549 and MDA-MB-231. B Clustering of RNA-seq data in MDA-MB-231 and BT549 with shCHES1. Significance was set based on padj < 0.05 and absolute fold-change > 2. C Venn diagram showed that 322 genes shared differentially expressed in both MDA-MB-231 and BT549 cells. D Heatmap showed the gene expression pattern of the 322 genes in both cell lines. E qPCR assay evaluated the mRNA levels of genes associated with CHES1 in BT549 and MDA-MB-231 cells. *p < 0.05, **p < 0.01, ***p < 0.001. F Western blot detected the protein levels of factors associated with CHES1 in BT549 and MDA-MB-231 cells. G Schematic representation of the CHES1 binding motif and potential binding sites (red triangle) on the upstream of MMP9, SNAI1, and PDGF-D transcription start site. The data were analyzed and downloaded from the JASPAR dataset (https://jaspar.genereg.net/). H The enrichment of CHES1 in the promoter region of genes were evaluated by ChIP. *p < 0.05, **p < 0.01, ***p < 0.001.

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