Fig. 2: GSDME-mediated pyroptosis and activation of apoptosic caspase-8/-3 were present in GM of aged mice with sarcopenia.
From: TNF-α contributes to sarcopenia through caspase-8/caspase-3/GSDME-mediated pyroptosis
A Representative immunoblots of GSDMD, N terminus of GSDMD(GSDMD-N), GSDME, N terminus of GSDME(GSDME-N), caspase (Casp)-8, cleaved-Casp(Cl-Casp)-8, Casp-3 and Cl-Casp-3 in GM of mice at different ages. B, C Relative expression levels of GSDMD-N, GSDME-N, Cl-Casp-8, and Cl-Casp-3 normalized to GAPDH based on densitometric analysis of immunoblots. D TUNEL staining, immunofluorescence staining of GSDME and immunohistochemical staining of Cl-Casp-8 and Cl-Casp-3 in GM of mice at different ages. Scale bar: 50 μm. E positive rates of TUNEL staining in the mice at different ages. F quantitation of immunohistochemical analyses for Cl-Casp-8 and Cl-Casp-3. G, correlation analysis of the relative expression level of GSDME-N with relative grip strength and GMI in mice. Data are expressed as mean ± SD. *P < 0.05, ***P < 0.001, ns: no significance. n = 8 mice per group.
