Fig. 1: HDGF promoted the malignant phenotype of NSCLC cells. | Cell Death Discovery

Fig. 1: HDGF promoted the malignant phenotype of NSCLC cells.

From: HDGF promotes gefitinib resistance by activating the PI3K/AKT and MEK/ERK signaling pathways in non-small cell lung cancer

Fig. 1

HDGF was knocked down by the CRISPR/Cas9 system in H1975 cells, while it was overexpressed in PC-9 and H292 cells by transfecting the plenti6-TR plasmid. HDGF expression was detected by Western blotting (A) and qRT‒PCR. B Proliferation of NSCLC cells with different levels of HDGF expression (C) or stimulated with 5 ng rhHDGF (D) was detected at 24, 48, 72 and 96 h. E Colony formation abilities of NSCLC cells with different HDGF expression levels. F Shows the analytical results of E. G The migration and invasion abilities of HDGF-knockdown H1975 cells were determined by Transwell assay. H Shows the analytical results of (G). I Migration and invasion abilities were determined in HDGF-overexpressing PC-9 and H292 cells. J shows the analytical results of (I). Data are from one representative experiment performed in triplicate. *P < 0.05, **P < 0.01, ***P < 0.005 vs. control.

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