Fig. 3: NETs upregulated TF expression in endothelial cells. | Cell Death Discovery

Fig. 3: NETs upregulated TF expression in endothelial cells.

From: Neutrophil extracellular traps contribute to immunothrombosis formation via the STING pathway in sepsis-associated lung injury

Fig. 3

A Representative images of control and NET-treated HUVECs. Scale bar: 100 μm. B Representative immunofluorescence staining of the cytoskeleton in control and NET-treated HUVECs. Scale bar: 100 μm. C The cell viability of HUVECs treated with NETs (n = 5). D The cellular supernatant LDH level was evaluated using a cytotoxicity detection LDH kit (n = 5). E Western blot images of TF expression in control and NET-treated HUVECs. F Relative mRNA levels of TF in control and NET-treated HUVECs (n = 3). G Representative images of immunofluorescence staining of TF in HUVECs. Scale bar: 100 μm. H Western blot images of TF expression in HUVECs with treatments. Each bar represents the mean ± SD. The comparison between the two groups was performed using unpaired t-tests (C–F). Statistical analysis for three or more groups was carried out using 1-way ANOVA (H). *p < 0.05, **p < 0.01, ***p < 0.001.

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