Fig. 5: NETs promoted STING upregulation by activating TLR2 signaling. | Cell Death Discovery

Fig. 5: NETs promoted STING upregulation by activating TLR2 signaling.

From: Neutrophil extracellular traps contribute to immunothrombosis formation via the STING pathway in sepsis-associated lung injury

Fig. 5

A KEGG analysis identified significantly altered pathways in control and NET-treated HUVECs. B Relative mRNA levels of TLRs in control and NET-treated HUVECs (n = 3). C Western blot images of TLRs expression in control and NET-treated HUVECs. D Representative images of immunofluorescence staining of TLR2 in control and NET-treated HUVECs. Scale bar: 30 μm. The expression of TLR2 was assessed by fluorescence intensity (n = 3). E The cell viability of HUVECs pretreated with C-29 (n = 3). F Relative mRNA levels of TLR2, STING, and TF in HUVECs pretreated with C-29 (n = 3). G Western blot images of STING pathway and TF expression in HUVECs pretreated with C-29. H Relative mRNA levels of STING in HUVECs transfected with LV-NC-RNAi or LV-TLR2-RNAi (n = 3). I Western blot images of TLR2 expression in HUVECs transfected with LV-NC-RNAi or LV-TLR2-RNAi. J Western blot was performed to analyze the levels of STING and TF expression in HUVECs transfected with LV-NC-RNAi or LV-TLR2-RNAi under stimulation of NETs. K Western blot images of TLR2 expression in HUVECs pretreated with DNase I. L Western blot images of TLR2 expression in murine lung tissues treated with DNase I. Each bar represents the mean ± SD. The comparison between the two groups was performed using unpaired t-tests (B, D, and H). Statistical analysis for three or more groups was carried out using 1-way ANOVA (E and F). *p < 0.05, **p < 0.01, ***p < 0.001.

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