Fig. 7: KRAS is a downstream target of miR-204-3p. | Cell Death Discovery

Fig. 7: KRAS is a downstream target of miR-204-3p.

From: Circular RNA hsa_circ_0001846 facilitates the malignant behaviors of pancreatic cancer by sponging miR-204-3p and upregulating KRAS expression

Fig. 7

A Schematic illustration of the wild-type (WT) and mutant (Mut) KRAS luciferase vectors. B The luciferase activities of the KRAS luciferase reporter vector (WT or Mut) in HEK293T cells transfected with miR-204-3p mimic or mimic NC. C The mRNA expression of KRAS was measured by qRT-PCR in PANC-1. D The protein level of KRAS was detected by western blot in PANC-1 transfected miR-204-3p inhibitor. E, F Designing and synthesizing the three small interference RNAs of KRAS and detecting the silencing efficiency in PANC-1 by qRT-PCR and western blot. G Expression level of KRAS was detected by western blot. All data are shown as the mean ± SD of at least three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001.

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