Fig. 4: Slowing of synaptic vesicle endocytosis by partial inhibition resulted in enhanced Aβ42 synaptotoxicity.
From: A delay in vesicle endocytosis by a C-terminal fragment of N-cadherin enhances Aβ synaptotoxicity
A–C Synaptotoxicity induced by short-term application of synthetic Aβ42 (1 µM) for 2 days to cultured cortical neurons was monitored by patch-clamp recordings of AMPA mEPSCs. L-685,485 (5 µM) or dynasore (20 µM) were co-applied to partially inhibit synaptic vesicle endocytosis (resulting in slower kinetics). A Example traces of AMPA mEPSCs recorded at −60 mV holding potential in the presence of 1 µM TTX and 10 µM gabazine, and under the experimental conditions indicated in front of each trace. B Quantitative analysis of AMPA mEPSC frequencies. Left: data for individual cells; n = 10/11/10/13/11/11 cells. Right: means ± SEM. C Quantitative analysis of AMPA mEPSC mean amplitudes. Note that the combination of two non-toxic conditions resulted in an enhanced synaptotoxicity. One-way ANOVA with Tuckey’s post hoc test. **P < 0.01.
