Fig. 2: MK2206 induced ferroptosis in CRC cells. | Cell Death Discovery

Fig. 2: MK2206 induced ferroptosis in CRC cells.

From: Targeting AKT induced Ferroptosis through FTO/YTHDF2-dependent GPX4 m6A methylation up-regulating and degradating in colorectal cancer

Fig. 2

A–C HCT-116 and SW480 were cultured 24 h with 0, 5 and 10 μM of MK2206, then GPX4 and β-Actin expression levels were detected by western blotting. D, E The expression levels of AKT, GPX4 and β-Actin were detected after AKT knockdown. F–H ROS positive cells as assessed by DCFH-DA fluorescence in HCT-116 and SW480 were treated with MK2206(10 μM), RSL3(2 μM), MK2206 + FER-1(4 μM) and MK2206 + NAC(20 μM), and their representative images obtained by a fluorescence microscope[scale bar, 100 μm].

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