Fig. 5: Ru/5-FU inhibits CRC stem cells. | Cell Death Discovery

Fig. 5: Ru/5-FU inhibits CRC stem cells.

From: A novel ruthenium complex with 5-fluorouracil suppresses colorectal cancer stem cells by inhibiting Akt/mTOR signaling

Fig. 5

A Representative images and (B) quantification of the number of colonies formed from HCT116 cells after treatment with Ru/5-FU. C, D Quantification of CD133 expression in HCT116 cells cultured in a monolayer after 24 h of incubation with 4 μM Ru/5-FU, as determined by flow cytometric analysis. E Representative confocal images of colonospheres formed from HCT116 cells after 24 h of incubation with 4 μM Ru/5-FU. The cells were stained with acridine orange (AO, green cells) and propidium iodide (PI, red cells that represent dead cells). Scale bar = 50 μm. F, G Quantification of CD133 expression in HCT116 cells cultured in colonospheres after 24 h of incubation with 4 μM Ru/5-FU, as determined by flow cytometric analysis. The vehicle (0.2% DMSO) was used as a control (CTL). Data are shown as the mean ± S.E.M. of three independent experiments carried out in duplicate. *P < 0.05 compared to CTL by Student’s t test or one-way ANOVA followed by Dunnett’s multiple comparisons test.

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