Fig. 3: Hsp27 improves mitochondrial function in hypoxic-reoxygenated cardiomyocytes. | Cell Death Discovery

Fig. 3: Hsp27 improves mitochondrial function in hypoxic-reoxygenated cardiomyocytes.

From: Upregulation of Hsp27 via further inhibition of histone H2A ubiquitination confers protection against myocardial ischemia/reperfusion injury by promoting glycolysis and enhancing mitochondrial function

Fig. 3

A, B Heat map depicting differentially expressed genes of the MAPK signaling pathway in cardiac tissue between the sham and MI/RI groups. Color-coded representation of gene expression levels: Green and red represented low and high expression, respectively. Data are representative of three independent experiments. B Representative images of JC-1 fluorescence in the H-R cardiomyocytes. C Detection of cellular ROS and lipid peroxide levels in the cardiomyocytes. D Statistical analysis of mitochondrial ROS levels. E Cellular MDA levels in cardiomyocytes. F Relative mRNA levels of ptgs2 in cardiomyocytes determined using qRT-PCR. G, H Viability of cardiomyocytes in the different groups. Data are presented as mean ± SD (n = 6). #P < 0.05 vs. Control group; *P < 0.05 vs. Reoxygenation group. Scale bars: [C (top)] 50 μm; [B, C (bottom)] 25 μm; and (G) 100 μm.

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