Fig. 5: Alteration of Bach1 expression and ferroptosis in astrocytes after high-altitude TBI. | Cell Death Discovery

Fig. 5: Alteration of Bach1 expression and ferroptosis in astrocytes after high-altitude TBI.

From: High-altitude hypoxia aggravated neurological deficits in mice induced by traumatic brain injury via BACH1 mediating astrocytic ferroptosis

Fig. 5

A–C Western blotting detection of the protein level of Bach1 (one-way ANOVA, F = 17.16, LAT vs LAS: *P = 0.0407; HAT vs HAS: #P = 0.0041; HAT vs LAT: &P = 0.0309), Fth1 (one-way ANOVA, F = 24.24, LAT vs LAS: *P = 0.0310; HAT vs HAS: #P = 0.0004; HAT vs LAT: &P = 0.0207) in the LAS, LAT, HAS and HAT groups (per group n = 3). D–F Representative images in astrocytes of different groups demonstrated Bach1 (one-way ANOVA, F = 124.3, LAT vs LAS: *P < 0.0001; HAT vs HAS: #P < 0.0001; HAT vs LAT: &P < 0.0001) was significantly increased and the ferroptosis marker Fth1 (one-way ANOVA, F = 159.7, LAT vs LAS: *P < 0.0001; HAT vs HAS: #P < 0.0001; HAT vs LAT: &P < 0.0001) was significantly decreased in high-altitude TBI (per group n = 3). G Tissue ROS intensity (one-way ANOVA, F = 15.62, LAT vs LAS: *P < 0.0260; HAT vs HAS: #P < 0.0036; HAT vs LAT: &P < 0.0253) in the LAS, LAT, HAS and HAT groups (per group n = 4). Scale bar: 20 μm. Data were shown as mean ± SD.

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