Fig. 2: Normal organogenesis and vascular development in the setd2-null zebrafish. | Cell Discovery

Fig. 2: Normal organogenesis and vascular development in the setd2-null zebrafish.

From: setd2 knockout zebrafish is viable and fertile: differential and developmental stress-related requirements for Setd2 and histone H3K36 trimethylation in different vertebrate animals

Fig. 2

a Normal development of the major organs as indicated by whole mount in situ mRNA hybridization with specific markers (written in parentheses) in the embryos of indicated genotypes at 3 dpf. The numbers of embryos used in each staining experiment and of those showed the presented patterns are labelled as denominators and numerators, respectively. b Normal vascular development as indicated by confocal microscopy images of flk1:EGFP-labelled blood vessels in the trunk of the heterozygous and homozygous setd2 mutants at 50 and 72 hpf. c RT-qPCR analysis of the expression levels of representative vasculogenic genes, whose mouse orthologs have been known to be up- and downregulated in Setd2 knockout mice19. The analyzed setd2 mutant zebrafish embryos were at 72 hpf. In b, c, the embryos were produced by a cross between heterozygous and homozygous zebrafish so that the WT could only be analyzed separately and therefore was not shown. **P < 0.01; n.s., not significant.

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