Fig. 5: Interaction interface in the CASK/Mint1 complex.
From: CASK modulates the assembly and function of the Mint1/Munc18-1 complex to regulate insulin secretion
a A surface representation of the CASK/Mint1 complex showing that Mint1-CID wraps around CASK-CaMK. The interaction interface between CASK and Mint1 can be divided into two sites (sites I and II, highlighted by dashed boxes). b, d An “Open-book” view of the two interaction sites of the CASK/Mint1 complex by a surface representation. Site I is contributed by the helical packing between the N-lobe of CASK-CaMK and the N-CID of Mint1 (b), and site II is mainly contributed by the interaction interface between the C-lobe of CASK-CaMK and the C-CID of Mint1 (d). In the drawing, the hydrophobic, positively charged, negatively charged residues and remaining residues are colored in yellow, blue, red and white, respectively. c, e A combined ribbon-and-stick model showing the interface in site I (c) and site II (e). The side chains of the key residues involved in the interface packing are shown as sticks. f–h Interaction between CASK-CaMK (residues 1–337) and Mint1-CID (residues 338-397) mutants measured by ITC assay: (f) I352A I355A I359A; (g) W381A and (h) 4A.
