Fig. 4: KRAS activation reduces α-KG levels in the cytosol and nucleus of PDAC cells. | Cell Discovery

Fig. 4: KRAS activation reduces α-KG levels in the cytosol and nucleus of PDAC cells.

From: The Fe–S cluster assembly protein IscU2 increases α-ketoglutarate catabolism and DNA 5mC to promote tumor growth

Fig. 4

a PaTu-8988t cells were transfected with a control siRNA, IscU2 siRNA, or KRAS siRNA. Cytosolic and nuclear α-KG levels were determined and normalized to the numbers of cells (means ± SEM, n = 3). b, c PaTu-8988t cells were transfected with a control siRNA, ACO2 siRNA, or DLST siRNA (b). PaTu-8988t cells were transfected with a control siRNA, KRAS siRNA, or KRAS siRNA with a vector expressing IscU2 (c). Both cytosolic and nuclear α-KG levels were determined and normalized to the number of cells (means ± SEM, n = 3). d, e HEK293T cells stably overexpressed KRAS G12V. Cytosolic (d) and nuclear (e) α-KG levels were determined and normalized to the numbers of cells (means ± SEM, n = 3). Statistical significance was determined by unpaired two-tailed Student’s t-test, ns not significant.

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