Fig. 3: Assembly of Tektin proteins in sperm axoneme DMT.
From: In-cell structural insight into the stability of sperm microtubule doublet
a A transverse section view of DMTF48 model to show the Tektin bundle in the A-tubule with Tektin proteins labeled. The coloring schemes are summarized in Supplementary Table S5. b Parallel comparisons of four pairs of Tektin1/2/3/4 filaments in each 48 nm repeat. The relative position of each pair in the Tektin1/2/3/4 bundle is shown and colored aside left. The sequential tubulins of the protofilament A08 form a 48 nm axis along the sperm axoneme with the coordinates from T0 to T12 and the direction from minus-end to plus-end of microtubule. The direction of Tektin filament is defined as the one from the C-terminus to N-terminus of one Tektin protein. The junction sites of Tektin filaments are indicated with black triangles and labeled with the coordinates Tx(+/−). Tx(+) means the site locates right to Tx and Tx(−) means left to Tx. c Parallel comparisons of Tektin5 proteins in each 48 nm repeat along with the axis defined by tubulins and with the junction sites labeled accordingly. Their positions in the Tektin bundle are also shown and colored aside left. d The distribution of junction sites of Tektin proteins in each 48 nm repeat.
