Fig. 6: The H2A-binding region of ZNHIT1 is required for histone exchange in cells.
From: Structural insights into histone exchange by human SRCAP complex
a Schematic diagram of establishing ZNHIT1-dTAG DLD-1 cells and validation of ZNHIT1 degradation and rescue. An empty vector, WT and mutant ZNHIT1 were transfected into the ZNHIT1-dTAG cells, respectively. The expression of target proteins was detected by western blot. Complex composition was validated by silver staining of the SRCAP-C purified from the indicated ZNHIT1-dTAG cells. b Metaplot and boxplot representation of H2A.Z occupancy in ZNHIT1-dTAG cells treated with DMSO or dTAG for the indicated times. c Metaplot and boxplot representation of H2A.Z occupancy in DMSO/dTAG-treated ZNHIT1-dTAG cells with overexpression of WT or mutant ZNHIT1. d Representative track examples showing H2A.Z occupancy in DMSO/dTAG-treated ZNHIT1-dTAG cells with overexpression of WT or mutant ZNHIT1. e Heatmaps of H2A.Z occupancy centered at TSSs of promoters ranked by decreasing occupancy in DMSO/dTAG-treated ZNHIT1-dTAG cells with overexpression of WT or mutant ZNHIT1.
