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Data availability
All relevant data are available from the authors and/or included in the manuscript. Atomic coordinates and EM density maps of the human ABCD4 have been deposited in the Protein Data Bank (PDB code: 6JBJ) and the Electron Microscopy Data Bank (EMDB code: EMD-9791), respectively.
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Acknowledgements
We thank Prof. Nieng Yan at Princeton University for help with establishment of our membrane protein research platform. We thank Xiaojun Huang and Boling Zhu at the Center for Biological Imaging, Core Facilities for Protein Science at the Institute of Biophysics, Chinese Academy of Sciences for technical supports during cryo-EM image acquisition, and Peiping Tang at the Center for Integrative Imaging, Hefei National Laboratory for Physical Sciences at the Microscale, University of Science and Technology of China for cryo-EM sample examination. This work is supported by the Ministry of Science and Technology of China (2015CB910103), the Chinese Academy of Science (XDB08020304), the National Natural Science Foundation of China (31621002), and the Fundamental Research Funds for the Central Universities (WK2070000117).
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C.-Z.Z. and Y.C. conceived the project and planned the experiments. D.X. expressed and purified human ABCD4. D.X., Z.F., and W.-T.H. performed functional assays. Z.F. performed initial negative-stain and cryo-EM analyses and prepared all grids. D.X. and Z.F. performed cryo-EM data collection. D.X. performed ABCD4 structure determination and Z.F. built the ABCD4 model. Y.-L.J. and L.S. revised the model, and Z.F. performed model refinement. D.X., Z.F., L.S., C.-Z.Z., and Y.C. wrote the manuscript; all authors contributed to the revision.
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Xu, D., Feng, Z., Hou, WT. et al. Cryo-EM structure of human lysosomal cobalamin exporter ABCD4. Cell Res 29, 1039–1041 (2019). https://doi.org/10.1038/s41422-019-0222-z
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DOI: https://doi.org/10.1038/s41422-019-0222-z
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