Fig. 3: ALR1 phosphorylates RbohD boosting STOP1 accumulation.
From: The LRR receptor-like kinase ALR1 is a plant aluminum ion sensor
a in vitro phosphorylation of RbohD in response to different concentrations of Al (0, 1, 10, 20 and 100 nM) detected with pS39 antibodies. CBS indicates coomassie blue staining. b in vivo phosphorylation of RbohD in seedlings following control or Al (50 µM) treatments. c, d Root growth under control and Al (1 mM) treatments (c), and their relative quantification (d) (n = 20). The average length of each genotype was set to 100%, and the relative root length was expressed as a percentage (root length with Al treatment/root length without Al × 100). e, f GFP-STOP1 fluorescence signals in roots (e) and their relative quantification under Al treatment (f) (n = 20). Bars = 1 cm (c), 100 µm (e). Data were analyzed by unpaired t-test (d, f) (ns non-significance, ****P < 0.0001).
