Fig. 5: ROS modify RAE1 to facilitate STOP1 accumulation.
From: The LRR receptor-like kinase ALR1 is a plant aluminum ion sensor
a in vitro labeling of BIAM for recombinant His-RAE1. b in vivo labeling of BIAM for FLAG-RAE1 immuno-precipitated from seedlings following 20 min control and Al (50 µM) treatments. c STOP1-GFP expression in mesophyll protoplasts with or without co-expressing RAE1 or mutated RAE1 (RAE1C364A). d Abundance of STOP1-GFP in protoplasts detected by α-GFP antibody. e Ubiquitination of STOP1-GFP in protoplasts detected by α-Ub antibody. f, g GFP-STOP1 fluorescence signals in roots (f) and their relative quantification under control condition (g) (n = 20). Bars = 100 µm (c, f). Data were analyzed by unpaired t-test (ns non-significance, ****P < 0.0001).
