Fig. 2: Ca²⁺ states and state-switching dynamics in SCN neurons.

Data were from a slice containing 6049 identified neurons. a Representative 5-min Ca²⁺ recordings, corresponding to different Ca²⁺ states of SCN neurons (State I to State VI). Data shown are Z-score normalized fluorescence intensity. b, c Phase-space manifolds (b) and recurrent plots (c) of corresponding traces in a. Note the repeating features or motifs of systematic dynamics in the recurrent plot. d Percentage of neurons at different Ca²⁺ states over a 24-h timescale. See Supplementary information, Fig. S4 for neuronal Ca²⁺ state classification. e Spatial distribution of neuronal Ca²⁺ states at CT28. The most populous State VI neurons (inset, top right) are omitted for clarity. f Raster plot of state-switching over 24 h for all neurons. The color codes of Ca²⁺ states are shown on the right.