Fig. 1: Cryo-EM structure of the chromatin fiber reconstituted with 12× 177 bp_601 DNA and linker histone H5. | Cell Research

Fig. 1: Cryo-EM structure of the chromatin fiber reconstituted with 12× 177 bp_601 DNA and linker histone H5.

From: Structural basis for linker histone H5–nucleosome binding and chromatin fiber compaction

Fig. 1

a The 3D reconstructed cryo-EM map of the H5-chromatin fiber at 3.6 Å resolution was viewed from two different angles. Three tetranucleosomal structural units are highlighted by different colors. b The atomic model of H5-chromatin fiber is viewed from two different angles. For viewing purposes, DNA is shown in partly transparent surface mode and colored as in a. Histone proteins are shown in cartoon representation with a color scheme as in c. c The dodecanucleosomal H5-chromatin fiber structure shows the ___location of linker histone H5 and a 1:1 stoichiometric association of H5:NCP. For clarity, only the DNA and H5 moieties are shown. The inset shows details of H5 in the dashed area. d Atomic structure of a representative chromatosome of H5-chromatin fiber, N7, viewed from two angles. The first and last resolved residues of each histone in N7 are labeled.

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