Fig. 7: Knockdown of LAMP-2A improves the therapeutic effects of MSCs on ConA-induced inflammatory liver injury.

Mice were intravenously injected with ConA (20 mg/kg) or PBS as a negative control, and then SCR-MSCs and L2A-KD-MSCs were transfused immediately. Eight hours later, the livers, spleens, and serum were sampled. a Hematoxylin and eosin staining of liver sections. The percentages of necrotic areas in the livers were calculated (n = 5 mice per group). b The weight of the livers was measured (n = 5 mice per group). c, d Serum levels of ALT and AST were measured by the enzyme-linked immunosorbent assay (n = 5 mice per group). e, f The percentages of CD8⁺ and CD4⁺ T cells in the liver and spleen were determined by flow cytometry, and the statistical results are shown (n = 5 mice per group). Scale bar: 500 μm. The results are presented as the mean ± s.e.m. Significant differences were analyzed by one-way ANOVA a–f and are expressed as *P < 0.05, **P < 0.01, ***P < 0.001, and n.s., no significance.