Fig. 3 | Cellular & Molecular Immunology

Fig. 3

From: The BCL-2 inhibitor APG-2575 resets tumor-associated macrophages toward the M1 phenotype, promoting a favorable response to anti-PD-1 therapy via NLRP3 activation

Fig. 3

APG-2575 remodels the transcriptomic landscape of macrophages and CD8+ T cells and promotes CD8+ T-cell infiltration via the chemokines CCL5 and CXCL10. A UMAP plot from merged data of tumor-infiltrating Mo/MF populations. B UMAP plots with annotated clusters of Mo/MF cells from the control and APG-2575 groups. C A heatmap showing the differentially expressed genes (rows) among Mo/MF subpopulations (columns). Representative genes from each cluster are highlighted (right). D Ratios of the proportions of Mo/MF clusters across different regimens. E GSEA using genes differentially expressed between IL-4-activated RAW264.7 cells with control or APG-2575 treatment. F‒I Scatter plot showing the results of Pearson correlation analysis of CCL5 and CXCL10 expression and the infiltration of CD8+ T cells and M1 macrophages in TCGA cohorts. J Growth of LLC tumors in C57BL/6 mice treated with the indicated regimens. (K–M) Flow cytometric analysis of CD8+, CD8 + GZMB+ and CD8+ TNF-α+ T cells in C57BL/6 mouse xenograft tumors treated with the indicated regimens. N‒Q Immunohistochemical staining of CD8 and GZMB in C57BL/6 mouse xenograft tumors

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