Fig. 1

RNA-seq identifies differentially expressed RBPs. a A schematic representation of the procedure used to prepare the samples for the RNA-seq analysis (left). CD34⁺ HPCs obtained from cord blood were differentiated into either the monocyte or granulocyte lineage, and cells were collected at 5, 10 and 15 days for the RNA-seq analysis. Temporal morphological changes (May-Grunwald Giemsa staining) in differentiated HPCs (Right). Magnification, 40×. b The workflow for differential expression (DE) RBP analysis. c Overlap of the global differentially expressed genes in b (left) or differentially expressed RBP genes (right) between batch 1 and batch 2. d Left panel: heat map showing temporal expression of genes during monocytic or granulocytic differentiation of CD34⁺ HPCs in duplicate samples from batch 1 and batch 2. Right panel: K-means clustering of the temporal profiles of the differentially expressed genes (DEGs) divided into seven individual clusters. RBP genes are shown as blue lines. e RNA-seq data for potential RBP genes that regulate myeloid differentiation. ‘Up’ or ‘Down’ represent a gene that is monotonically up-regulated or downregulated in the corresponding culture, respectively, whereas ‘EE’ indicates a gene that is not a DEG in this cell culture. f qPCR analysis of M-CSFR, CD14, ITGAM and SP1 mRNA expression during monocyte differentiation and ITGAM, G-CSFR, CEBPA and MPO mRNA expression during granulocyte differentiation. Up-regulated genes were shown in red and downregulated gene was shown in green. Three technical replicates from a single experiment. g qPCR analysis of the expression of the eight candidate RBP mRNAs during monocyte and granulocyte differentiation. Three technical replicates from a single experiment. Data are shown as means±s.d.