Fig. 2: NOX2 deficiency decreases inflammatory response in CD11b⁺F4/80^low macrophages in mice fed a high-fat diet.

WT and Nox2 KO mice were fed a high-fat diet for 12 weeks. a, b Isolated whole liver MNCs were subjected to flow cytometry analyses. c The generation of ROS was monitored by DCF fluorescence in freshly isolated CD11b⁺F4/80^high Kupffer cells and CD11b⁺F4/80^low macrophages. d, e Whole liver MNCs and tissues were subjected to qRT–PCR and Western blotting, respectively. f Apoptotic bodies were assessed and counted after TUNEL staining (average number of 5 fields under ^χ200 magnification). Bar = 200 μm. g After treatment with palmitate for 1 h, CD11b⁺F4/80^low macrophages stained with Annexin V and 7-AAD were analyzed by flow cytometry. Data are representative of three independent experiments using 5 (a–f) and 3 (g) mice per group. Data are expressed as the mean ± s.e.m. and analyzed by Student’s t-test, **P < 0.01 in comparison with the corresponding controls