Fig. 6
From: Midbrain circuit regulation of individual alcohol drinking behaviors in mice
VTA-NAc neurons of alcohol drinking mice have different intrinsic properties and distinct responses to EtOH. a Timeline of experiments and schematic of surgery. b Representative Ih traces (left) and I–V graph of Ih (right). (two-way RM ANOVA: drinking group effect F(2, 306) = 4.442, P < 0.05; interaction effect F(12, 306) = 5.421, P < 0.0001. Bonferroni post hoc test *P < 0.05; **P < 0.01; ***P < 0.001. n = 23 cells from 7 mice; n = 10 cells from 5 mice; n = 21 cells from 6 mice). c Representative spike traces after current injection (left, 150 pA) and excitability data of VTA-NAc neurons from EtOH naive, low alcohol drinking, and high alcohol drinking mice (two-way RM ANOVA: drinking group effect F(2, 159) = 4.551, P < 0.05; interaction effect F(6, 159) = 1.839, P = 0.0948. Bonferroni post hoc test *P < 0.05. n = 21 cells from 5 mice; n = 10 cells from 3 mice; n = 25 cells from 5 mice). Data represented as mean + S.E.M. d Representative spontaneous firing rate traces during different bath applications of EtOH (left) and % change (Δ) in firing rate from baseline (responsivity) during bath application of 20, 50, and 100 mM EtOH in aCSF (right) (two-way RM ANOVA: drinking group effect F(2, 64) = 15.18, P < 0.0001; EtOH concentration effect F(2, 64) = 12.67, P < 0.0001; interaction effect F(4, 64) = 10.30, P < 0.0001. Bonferroni post hoc test ***P < 0.001. n = 10 cells from 6 mice; n = 11 cells from 3 mice; n = 14 cells from 7 mice). Data represented as mean + S.E.M.
