Fig. 3

mTORC1 inhibition by rapamycin inhibits RPC cell cycle and retinal development. a Pregnant mice were injected with vehicle (see Methods) or rapamycin (5 mg/kg) into the peritoneum at 12 dpc, and the embryos were collected at 12.5 dpc (E12.5). BrdU were injected at 3 h prior to the embryo collection. Distribution of each marker in the embryonic retinas was investigated by immunostaining with corresponding antibodies. Dotted lines mark retinal margins. Scale bar, 100 μm. b Cell cycle progression of embryonic RPCs in the rapamycin-injected mice was examined as described in Fig. 2f. Scale bar, 100 μm. c Percentages of cells expressing the markers in a are shown in a graph. Values are averages (n = 5 from 3 independent litters) and error bars denote SD. d Ratios of BrdU-co-expressing cells to total marker-expressing cells are shown in a graph (n = 5 from 3 independent litters). Error bars denote SD. P-values are obtained by one-way ANOVA test and shown in the graphs (*<0.05; **<0.01; ***<0.001)