Fig. 1
TRPV1 closure induced by cooling in the presence of saturating concentrations of capsaicin. a Distribution of pore radii in the cryo-EM-derived closed state (3J5P) and capsaicin bound state (3J5R). The regions in red are too narrow to allow a water molecule to pass. The regions in green or blue are sites allowing single or multiple water molecules and ions to pass. Pore radii were calculated by the HOLE program65. SF, selectivity filter. b Temperature-dependent reduction of macroscopic current amplitude is steeper than that of single-channel conductance. Macroscopic currents from three inside-out patch recordings (shown in red, blue, and green) and single-channel current (shown in black) are normalized to the amplitude at 10 °C. Superimposed are linear fits of the single-channel conductance levels between 5 and 30 °C (black dashed line) or the macroscopic currents in the middle (red dashed line) and low (blue and green dashed lines) temperature ranges. c Example single-channel traces at representative temperatures in the presence of 10 μM capsaicin. d Representative consecutive open probability measurements of three single-channel recordings (green, blue, and orange circles), as well as the average Po (red diamonds) fitted to an exponential function (red dotted curve)
