Fig. 5
The conformational wave in capsaicin activation of TRPV1. a Measured Φ values were mapped on TRPV1 structure. The sites moved early (large Φ values) or late (small Φ values) were colored in red or blue, respectively. b The Φ values were clustered and averaged by their locations. Capsaicin-binding pocket (M548 and T551): 0.82 ± 0.08; S4–S5 linker (E571): 0.55; S6 bundle crossing (I680, L682, and E685): 0.59 ± 0.10; Selectivity filter (T642, I643, D647, L648, and E649): 0.31 ± 0.11. c fprogress values calculated by iENM were mapped on TRPV1. Residues moved early (small fprogress value) or late (large fprogress value) were colored in red or blue, respectively. d The Φ values (black) and fprogress values (orange) matched well with each other in multiple domains. e A schematic diagram showing the temporal sequence of events upon capsaicin binding that lead to the full activation of TRPV1. All statistical data are given as mean ± s.e.m
