Fig. 5
From: Precise tuning of gene expression levels in mammalian cells
CRISPR/Cas9-mediated miSFIT knockin enables fine-tuning of endogenous BRCA-1 expression. a Experimental workflow for tuning endogenous gene expression by genomic integration of miSFIT regulatory elements in the native 3′UTR of a target gene using CRISPR-mediated HDR. Assessing the impact of miSFIT variants is carried out in a pooled format by high throughout sequencing. b Impact of a panel of 17 miR-17 miSFIT variants on endogenous BRCA-1 expression. (n = 3 biological replicates, mean—s.d.). The position and identity of mismatched bases in each variant is indicated by the colour code. Bar plot reflects the degree of BRCA-1 repression imparted by each miSFIT variant relative to the scrambled MRE control. Source data are provided as a Source Data file
