Fig. 3
From: FOXF2 reprograms breast cancer cells into bone metastasis seeds
FOXF2 enhances the bone-specific metastasis potential of breast cancer cells. MCF-7 and MDA-MB-231 cells were treated as indicated. a Chemotactic migration of cancer cells toward MC3T3E1 or BEAS-2B cells was assessed by transwell assays. b Adhesion of cancer cells to MC3T3E1 or BEAS-2B cells was assessed by adding cancer cells to MC3T3E1 or BEAS-2B cells at 100% saturation, followed by incubation for 30 min. c Anchorage-independent growth of cancer cells in the CM from MC3T3E1 or BEAS-2B cells was assessed by soft agar colony-formation assays. d The ability of cancer cells to induce osteoclast differentiation was assessed by incubation of pre-induced primary preosteoclasts with cancer cell CM containing 50 ng ml–1 RANKL. The number and size of mature osteoclasts with TRAP-positive multinucleated (≥3 nuclei) were analyzed. Scale bars: 100 μm for (a–c) and 500 μm for (d). *P < 0.05 by Student’s t test. Error bars are defined as s.d.
