Fig. 4
From: ERAP1 promotes Hedgehog-dependent tumorigenesis by controlling USP47-mediated degradation of βTrCP
ERAP1 impairs Hh-dependent growth of cerebellar granule cell progenitors. a H&E and immunohistochemical staining of Ki67, ERAP1 and Gli1 in the outer EGL during mouse cerebellum development. Magnification ×40. Scale bars represent 50 μm. b, c GCPs were isolated from 4-day-old mice and treated with either SAG alone or in combination with increasing doses of Leu-SH for 24 h. BrdU uptake (b) and mRNA levels of Gli1 (c) are shown. d, i GCPs isolated from 4-day-old mice were infected with lentiviral particles encoding for shERAP1 (d–f) or ERAP1 (g–i) and the corresponding controls, respectively. The percentage of BrdU uptake (d, g), mRNA (e, h), and protein levels (f, i) of Gli1 are shown. Results in c, e, h were normalized to endogenous GAPDH and HPRT controls and expressed as described in Fig. 1 legend, and represent the mean of three independent experiments. In f and i, actin was used as loading control. Mean ± S.D. *P < 0.05; **P < 0.01 determined with two-sided Student’s t-test
