Fig. 4
From: Mitigation of off-target toxicity in CRISPR-Cas9 screens for essential non-coding elements
High-specificity CRISPR-Cas9 screen designs for non-coding elements. a Distribution of GuideScan specificity scores for two non-coding libraries from this study and a gene-targeting library, in comparison to all possible sgRNA. b Most TSSs can be targeted with multiple high-specificity sgRNA. Fraction of TSS in the ENCODE SCREEN database of ccREs that can be targeted with dCas9-based epigenome editors within a window of +/−100 bp, after filtering for GuideScan scores >0.2. c Fraction of motifs in TFBS motifs that can be targeted with sgRNAs with a cut site in the motif, after filtering out low-specificity sgRNAs
