Fig. 3

Vertebral lymphatic vessel connections with sympathetic ganglia. a, b Thoracic PROX1⁺ LVs contact paravertebral sympathetic ganglia (SG) (blue arrows). White dotted-lines: DRG. c PROX1 (white) and tyrosine hydroxylase (TH, red) double labeling shows a ventral LV branch contacting a paravertebral TH⁺ sympathetic ganglion (blue arrow). d–f PROX1 (white)/LYVE1 (red) double labeling of the LV-SG connection (blue arrow). g–i 2D-confocal images of cervical cryosections labeled with LYVE1 (white), TH (red), and DAPI (blue). White box: area magnified in (h) and (i). A LV contacts a TH⁺ SG (blue arrow). Note a second ventral LV branch running along the SG, without entering its cortical layer (h, salmon arrow). This branch is also seen in panel (b) (salmon arrow). White dotted-lines: DRG; Red asterisk: vertebral ventral body; SC: spinal cord. Scale bars: 100 µm (a–h); 200 µm (i)