Fig. 3
EZH2 directly targets the promoter of ERα gene (ESR1). a Under 3D culture conditions, MDA-MB-231 cells were treated with DMSO, dinaciclib (Dina) or EPZ-6438 (EPZ) for 3 days. The cells were collected to extract RNA. ERα expression was determined by real-time RT–PCR. Data were expressed as mean ± SEM (n = 6). b–d ERα target gene expressions were determined by real-time RT–PCR for RNA from Fig. 3a. Data represent mean ± SEM (n = 3). e Chromatin immunoprecipitation (CHIP) assay using anti-EZH2 antibody to detect binding of EZH2 to ERα promoter regions. The immunoprecipitates were isolated from 3D cultured cells treated with DMSO, Dinaciclib or EPZ-6438. Data are representative binding to ER promoter relative to input control (mean ± SEM, n = 3). f CHIP assay using anti-H3K27me3 antibody to detect binding of H3K27me3 to ER promoter regions. The immunoprecipitates were isolated from 3D cultured cells treated with DMSO, Dinaciclib or EPZ-6438. Data are binding to ER promoter relative to input control (mean ± SEM, n = 3). **p < 0.05; ***p < 0.01; ****p < 0.001, Student’s t test. Source data are provided as a Source Data file.
