Fig. 7: Mapping of the TRIB3-AKT1 interaction.

a Mapping AKT1 regions binding to TRIB3. Schematic diagram of full-length AKT1 and deletion mutants. b, c HEK293T cells were co-transfected with the indicated AKT1-Myc and TRIB3-HA constructs. Cell extracts were IP with an anti-HA Ab. d Mapping TRIB3 regions binding to AKT1. Schematic diagram of full-length TRIB3 and deletion mutants. e HEK293T cells were co-transfected with the indicated AKT1 and TRIB3-GFP constructs. Cell extracts were IP with an anti-Myc Ab. f Prediction of the AKT1 and TRIB3 interaction using Discovery Studio. g Kinetic interactions of α-helical peptides and the TRIB3 protein were determined by SPR analyses. h Schematic diagram of full-length AKT1 and deletion mutants. i The △Ae AKT1 mutant barely binds the TRIB3 protein. HEK293T cells were co-transfected with the indicated mutants of AKT1-Myc and TRIB3-HA. Cell extracts were IP with an anti-HA Ab. j Prediction of the Pep2–Ae and TRIB3 interaction by Discovery Studio. Source data are provided as a Source Data file.