Fig. 6: Regulation of T_SCM CD4 cells homeostasis during aging.

a Hyporesponsiveness of the Wnt/β-catenin pathway during aging. The frequency of specifically TWS119-induced T_SCM CD4 cells was evaluated by flow cytometry, and calculated after the subtraction of nonspecific DMSO-induced T_SCM CD4 cells frequencies. The statistical analysis was performed on unpaired samples (U Mann–Whitney test, ** for p < 0.01). Source data are provided as a Source Data file. b CD127 expression characterizes induced-T_SCM CD4 cells. The phenotype of induced-T_SCM CD4 T cells was performed at day 7. The statistical analysis was performed on paired samples (Wilcoxon signed-rank test) (* and ** for p < 0.05 and p < 0.01, respectively). Source data are provided as a Source Data file. c Identification of recent thymic emigrants in cord blood or in peripheral blood of young and old donors by flow cytometry. T_RTE were defined as PTK7⁺CD31⁺CD4 T cells. d Induction of T_SCM CD4 cells from T_RTE cells. The correlations between ex vivo T_RTE and in vitro TWS119-induced T_SCM CD4 cells at day 7 were calculated with the Spearman’s rank-order test (n = 15). Source data are provided as a Source Data file. e T_SCM CD4 cells induction depends on CD31 expression in naive CD4 T cells. Flow cytometry staining of TWS119 dose-dependent induced-T_SCM CD4 T cells derived from CD31⁻ or CD31^high naive CD4 T cells. f Increased potential of CD31^high naive to T_SCM CD4 cells differentiation. Frequencies of T_SCM CD4 cells derived from CD31⁻ or CD31^high naive CD4 T cells in response to the stimulation of the Wnt/β-catenin pathway with low dose of TWS119 (5 μM). Young and old donors are represented with black and gray symbols, respectively. The statistical analysis was performed on paired samples (Wilcoxon signed-rank test) (***for p < 0.001). Source data are provided as a Source Data file. g Phenotype of induced T_SCM from T_RTE and CD31^low naive CD4 T cells. Flow cytometry staining of induced-T_SCM CD4 cells derived from CD31⁻ or CD31^high (T_RTE) naive CD4 T cells. Histograms represented overlaid expression of individual markers after 7 days of culture in presence of vehicle alone (DMSO) or Wnt/β-catenin stimulating drug (TWS119) at 5 or 10 μM.