Fig. 3: Effects of FAM13A knockdown in human adipocyte differentiation.
From: FAM13A affects body fat distribution and adipocyte function
a FAM13A mRNA expression during adipogenesis of human SGBS preadipocytes. b mRNA expression of FAM13A, measured 2 days after siRNA transfection and before initiation of adipogenesis. c mRNA expression of adipogenic markers (CEBPA, PPARG), measured 5 days after adipogenic induction in cells transfected with scrambled siRNA or siFAM13A. d mRNA expression of FAM13A, measured 8 days after lentiviral infection and before initiation of adipogenesis. e mRNA expression of adipogenic markers (CEBPA, PPARG), measured 5 days after adipogenic induction in cells transduced with scrambled sgRNA or three independent sgRNAs against FAM13A. f mRNA expression of FAM13A, measured on D10 of adipogenesis and 2 days after siRNA transfection. g Basal and insulin-stimulated [3H] glucose uptake, measured on D12 differentiated adipocytes and 4 days after siRNA transfection. Data are presented as mean ± SEM. n = 3 independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001, unpaired t test (for b and f), one-way ANOVA followed by Turkey’s multiple comparison test (for a and d), 2-way ANOVA followed by Sidak’s multiple comparison test (for c). Source Data are provided as a Source Data file.
