Fig. 2: The loss of autophagy promotes resistance to T-cell-mediated anti-tumour activity in vivo. | Nature Communications

Fig. 2: The loss of autophagy promotes resistance to T-cell-mediated anti-tumour activity in vivo.

From: Autophagy deficiency promotes triple-negative breast cancer resistance to T cell-mediated cytotoxicity by blocking tenascin-C degradation

Fig. 2

a, b Tumour growth of mouse 4T1-WT or 4T1-Atg5KO#1 cells in BALB/c mice (n = 6 mice per group). Tumour volumes (left, a) and tumour weights upon autopsy on day 21 (right, a) were calculated. Representative TILs dot plots from a representative mouse for each group were shown (upper, b). The percentage of TILs for each group were calculated (bottom,b). c Tumour growth of 4T1-WT and 4T1-Beclin1KO#2 tumour xenografts in BALB/c mice following treatment with mouse anti-PD1 antibody. The treatment protocol was summarized by the arrows. d, e Tumour growth of 4T1-WT cells in BALB/c mice with anti-PD-1 antibody treatment (n = 6 mice per group). Tumour volumes (left, d) and tumour weights upon autopsy on day 18 (right, d) were calculated. Representative images of TUNEL staining (green) and DAPI-stained nuclei (blue) in xenograft tumour sections were shown (left, e). Quantification of positive TUNEL cells (right, e). f Tumour growth of 4T1- Beclin1KO#2 cells in BALB/c mice with anti-PD-1 antibody treatment (n = 6 mice per group). Tumour volumes (left, f) and tumour weights upon autopsy on day 18 (right, f) were calculated. g Tumour growth of 4T1-WT and 4T1-Atg5KO#1 tumour xenografts in BALB/c mice following treatment with mouse anti-PD-L1 antibody. The treatment protocol was summarized by the arrows. h–j Tumour growth of 4T1-WT cells in BALB/c mice with mouse anti-PD-L1 antibody treatment (n = 8 mice per group). Tumour volumes (left, h) and tumour weights upon autopsy on day 14 (right, h) were calculated. Representative TILs dot plots from a representative mouse for each group were shown (upper, i). The percentage of TILs for each group were calculated (bottom, i). Representative images of TUNEL staining (green) and DAPI-stained nuclei (blue) in xenograft tumour sections were shown (left, j). Quantification of positive TUNEL cells (right, j). k Tumour growth of 4T1-Atg5KO#1 cells in BALB/c mice with mouse anti-PD-L1 antibody treatment (n = 8 mice per group). Tumour volumes (left, k) and tumour weights upon autopsy on day 14 (right, k) were calculated. Error bars represent mean ± SEM. The P value was determined by a two-tailed unpaired Student’s t test. Data are representative of two independent experiments.

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