Fig. 8: Blockade of TNC sensitizes checkpoint blockade immunotherapy in vitro. | Nature Communications

Fig. 8: Blockade of TNC sensitizes checkpoint blockade immunotherapy in vitro.

From: Autophagy deficiency promotes triple-negative breast cancer resistance to T cell-mediated cytotoxicity by blocking tenascin-C degradation

Fig. 8

a MDA-MB-231-Atg5KO#4 cells were pre-treated with anti-TNC (10 µg per ml) or anti-PD-L1 (10 µg/ml) for 2 h, then co-cultured with CD3/CD28-activated human T cells. Left, representative dot plots of the cleavage of caspase-3 in tumour cells measured by flow cytometry. Right, percentage of cleaved caspase-3+ tumour cells. b MDA-MB-231-Atg5KO#4 cells were pre-treated with anti-TNC (10 µg/ml) for 2 h, then co-cultured with CD3/CD28-activated human T cells in the presence of nivolumab (10 µg/ml). Left, representative dot plots of the cleavage of caspase-3 in tumour cells measured by flow cytometry. Right, percentage of cleaved caspase-3+ tumour cells. c MDA-MB-231-Atg7KO#5 cells were pre-treated with anti-TNC (10 µg/ml) or anti-PD-L1 (10 µg/ml) for 2 h, then co-cultured with P53 antigen-specific activated human T cells. Upper, representative dot plots of the cleaved caspase-3 in tumour cells measured by flow cytometry. Bottom, percentage of cleaved caspase-3+ tumour cells. d MDA-MB-231-WT cells were pre-treated with 50 µM Resveratrol for 24 h, then co-cultured with CD3/CD28-activated human T cells. Upper, representative dot plots of the cleavage of caspase-3 in tumour cells measured by flow cytometry. Bottom, percentage of cleaved caspase-3+ tumour cells. Error bars represent mean ± SEM, n = 3 biological independent samples. The P value was determined by one-way ANOVA with the Dunnett’s multiple comparisons test, no adjustments were made for multiple comparisons. NS no significance. All data are representative of three independent experiments.

Back to article page